[email protected]

Blood Collection and Sample Preparation for Rodents

Serum Aqueous part of blood where fibrin and blood cells are removed by coagulation or with use of a serum separator tube. Serum can be obtained by placing whole blood in an empty tube and allowing the blood to clot or by placing in a specialized serum separator collection tube. Collection of Serum from Blood (Procedure) :Immunology Procedure Collect the blood into a glass container and allow it to clot at room temperature for 1h. Once the clot has formed, loosen it from the walls of the container to aid retraction. Keep at 4 o C and leave it there overnight. Collect the eed serum and centrifuge at 150 g for 5 min (to

PBMC and Monocyte Isolation from Whole Blood

Aliquot 600ul of serum from red top tube into pre-labeled serum tubes. 8.3.4 Store in -80°C Freezer. 8.4 Ficoll Gradient:8.4.1 Traditional method: Add 20mls of Ficoll to new 50mL conical replicating number of blood tubes. E.x. 2 blood tubes, 2 Ficoll tubes. Add up to 30mls 1X PBS to whole blood Quality Control of Serum and Plasma by Quantification of

  • AbstractMaterials and MethodsResultsDiscussion13 Nonstandard AbbreviationsBlood samples are one of the most frequently used materials in medical research and practice. Conclusions with wide-reaching consequences are drawn from investigations involving serum and plasma. Consequently, the quality of blood samples is of the utmost importance for valid diagnostics and appropriate therapeutic decisions, as well as for reliable, conclusive research findings. Indications of good-quality samples can be based on whether blood samples are collected and handled in a reprodBlood Fractionation Protocol for Collection of White Blood Fractionate the whole blood by centrifuging at 1500-2000 X g for 10-15 min at room temperature. This will separate the blood into an upper plasma layer, a lower red blood cell (RBC) layer, and a thin interface containing the WBCs (see Figure 1). Although the suggested procedure is to fractionate the blood as soon as possible after collection Specimen-handling, Serum, Plasma, Whole blood :Serum SERUM, PLASMA OR WHOLE BLOOD COLLECTION WHOLE BLOOD. Collect whole blood according to instructions provided for the individual test. Thoroughly mix the blood PLASMA. Plasma contains fibrinogen and other clotting factors when separated from the red blood cells. Evacuated tubes SERUM. Please

    Standard Operating Procedure (SOP) for

    6.2.2. Whole blood is collected in Na citrate CPT tubes. Record and report the time when it is collected. Store tubes in an upright position at room temperature (15 to 30°C) until centrifugation. Gently invert the tubes eight to ten times to remix cells immediately before centrifuging. Whole Blood Protocol NIDDK

    • PurposeBackgroundProcedure1. Calibration of whole blood creatinine method to a heparinized plasma IDMS-traceable method at variable hematocrit levels 1. A heparinized venous whole blood sample from a healthy volunteer should be collected in heparin anticoagulated tubes and determined to be within the adult reference ranges for the following analytes. 1. A portion of the whole blood sample should be centrifuged and the plasma removed. 2. Divide the remaining blood sample into three aliquots. Adjust an aliquot of whole blood to lSOP COLLECTION OF BLOOD - UrowebProcessing of Serum tubes (samples B01, B02):Rack the tubes upright at room temperature for 30 minutes (minimum 30 minutes, maximum 60 minutes) to encourage clotting. Invert the blood tubes gently (10 to 15 times) and then place them in the centrifuge. Centrifuge the Standard Operating Procedures for Serum and Plasma As noted above, in order to obtain serum of high quality, blood samples should be allowed time to form a clot at room temperature for 3060 min. 10 The contributed SOPs had a range of time to allow clot formation that was reasonably consistent at 30 min minimum to 60 min maximum (with one at up to 5 h), and the final SOP directs users to allow 3060 min to clot, then process in a centrifuge or hold at 4